SQSTM1 anticorps (N-Term)

N° du produit ABIN7603119

Cet anticorps Souris Monoclonal détecte spécifiquement SQSTM1 dans WB, IHC, IF, FACS et ICC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour SQSTM1 anticorps (N-Term) (ABIN7603119)

Antigène: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SQSTM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 3H11

Détail du produit anti-SQSTM1 anticorps

Épitope: N-Term

Fonction: Anti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) (ABIN7603119). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1/p62, identical to the related mouse and rat sequences.

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Hocking, L. J., Lucas, G. J. A., Daroszewska, A., Mangion, J., Olavesen, M., Cundy, T., Nicholson, G. C., Ward, L., Bennett, S. T., Wuyts, W., Van Hul, W., Ralston, S. H. Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease. Hum. Molec. Genet. 11: 2735-2739, 2002. 2. Joung, I., Strominger, J. L., Shin, J. Molecular cloning of a phosphotyrosine-independent ligand of the p56-lck SH2 domain. Proc. Nat. Acad. Sci. 93: 5991-5995, 1996. 3. Park, I., Chung, J., Walsh, C. T., Yun, Y., Strominger, J. L., Shin, J. Phosphotyrosine-independent binding of a 62- kDa protein to the src homology 2 (SH2) domain of p56-lck and its regulation by phosphorylation of ser-59 in the lck unique N-terminal region. Proc. Nat. Acad. Sci. 92: 12338-12342, 1995.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur SQSTM1

Antigène: SQSTM1 (Sequestosome 1 (SQSTM1))

Autre désignation: SQSTM1

Sujet:

Synonyms: Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, SQSTM1, ORCA, OSIL

Tissue Specificity: Ubiquitously expressed.

Background: SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.

Poids moléculaire: 62 kDa

ID gène: 8878

UniProt: Q13501

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Autophagy